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Biochemicals and Reagents > Fluorescent Probes, Labels, Particles and Stains > Detection
Fluorescent Probes, Labels, Particles and Stains > Fluorescent Labels > Fluorescein and Derivatives
Fluorescent Stains > Nucleic Acid Stains > Nucleic Acid Stains For Microscopy

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86826

Sigma

5-Carboxyfluorescein

BioChemika, for fluorescence, ≥95% (HPLC)


Synonym:	5-FAM
CAS Number:	76823-03-5
Linear Formula:	C₂₁H₁₂O₇
Molecular Weight:	376.32
Beilstein Registry Number:	57037
MDL number:	MFCD00036874
PubChem Substance ID:	24888769

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Description

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers^1,²

Properties

grade	for fluorescence
product line	BioChemika
assay	≥95% (HPLC)
fluorescence	λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0
solubility	DMF: soluble
	DMSO: soluble
	H₂O: soluble

Safety

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany	3

References

Cited Reference	1. Nucleic Acids Res. 20, 2471, (1992) Abstract
	2. Anal. Biochem. 223, 39, (1994) Abstract

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